Human Umbilical Cord Blood Mesenchymal Stem Cells Colonizing in Hypoxic-Ischemic Encephalopathy Rats Brain
- VernacularTitle:人脐带血间充质干细胞在缺氧缺血性脑病新生鼠脑内的定植
- Author:
sheng-ping, XIAO
;
lian, MA
;
min-jie, LUO
;
xue-wu, JIANG
;
tian-hua, HUANG
;
yan-ping, ZHANG
;
qing-dong, XIE
- Publication Type:Journal Article
- Keywords:
umbilical cord blood;
stem cell;
hypoxic-ischemic encephalopathy;
colonizing
- From:Journal of Applied Clinical Pediatrics
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore human umbilical cord blood mesenchymal stem cells(MSCs)colonization in hypoxic-ischemic encephalopathy(HIE)rats brain.Methods Models of 7-day-old newborn rats with HIE brain injury were established.Meanwhile,on the same day,MSCs were transplanted with Hoechst 33258 for 24 hours into rats models marked by flurescent nucleotide dye injected through caudal vein or with stereotactic instrument.After 15-30 days,then MSCs were detected with fluorescene microscope.Results With the improved rice methods,HIE animal model was successfully attained.Majority of MSCs were distributed in the cortex,hippocampus of the lesioned hemisphere,especially in the forehead.And abtained a good fusion with HIE rats brain tissue.Conclusion Human umbilical cord blood MSCs can be cultured,when transplanted into the HIE rats model,they can move into intracranial,and integer with rats brain tissue.
