Effect of Adenovirus Type 3I,7b on Expressions of mRNA and Protein of Transforming Growth Factor-Beta 1 in Human Embryonic Lung Fibroblast Cells
- VernacularTitle:3I、7b型腺病毒感染对人胚肺成纤维细胞转化生长因子?_1 mRNA及其蛋白表达的影响
- Author:
hua, XU
;
wen-ge, WANG
- Publication Type:Journal Article
- Keywords:
human embryonic lung fibroblast cells;
adenovirus type 3I,7b;
transforming growth factor-?_1;
enzyme-linked immunosorbent assay;
in situ hybridization
- From:Journal of Applied Clinical Pediatrics
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of adenovirus type 3I,7b on the expressions of mRNA and protein of transforming growth factor-beta 1(TGF-?1) in human embryonic lung fibroblast cells.Method The expression of mRNA and protein of TGF-?1 were determined in human embryonic lung fibroblast cells before and after being infected by adenovirus type 3I,7b and in normal fibroblast cells with enzyme-linked immunosorbent assay and in situ hybridization.Results The mRNA and protein of TGF-?1 expression in human embryonic lung fibroblast cells increased siginificantly after being infected by adenovirous type 3I,7b compared with those in normal fibroblast cells(Pa0.05).Conclusion Lung fibroblast cells and TGF-?1 may play some roles in pathophysiological processes of viral pneumonia.
