Construction of Microvessel Angiogenesis Model in Nonobese Diabetic/Severe Combined Immunodeficient Disease Leukemia Mice
- VernacularTitle:非肥胖糖尿病/重症联合免疫缺陷小鼠白血病微血管生成模型的建立
- Author:
hai-xia, GUO
;
yang, LI
;
yan-qin, ZOU
;
wen-yi, LI
- Publication Type:Journal Article
- Keywords:
angiogenesis;
animal model;
leukemia
- From:Journal of Applied Clinical Pediatrics
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct microvessel angiogenesis model in nonobese diabetic/severe combined immunodeficient disease(NOD/SCID) leukemia mice.Methods Divided NOD/SCID mice into group A:blank group;group B:endothelial cell(EC) injection group;group C:leukemia model mice with EC injection group.Microvessel density(MVD) in bone marrow was calculated with cnemis slice after immunohistochemistry dyeing.Evaluated leukemia burden in leukemia model mice.Results Bone marrow MVD in group C was significantly more than group B.Microvessels in leukemia mice had disordered branches and irregular cavity.The morphism of vessels was immature.Conclusions Microvessel angiogenesis model in NOD/SCID leukemia mice has been constructed successfully.It can be used in the study of anti-angiogenesis in leukemia and relative researches.
