Effects of Bone Marrow Mescenchymal Stem Cells Conditioned-Medium on Cortical Neuronal Apoptosis Induced by Hypoxia-Reoxygenation in vitro in Rats
- VernacularTitle:骨髓间充质干细胞条件培养基对缺氧大鼠神经元凋亡的影响
- Author:
cai-xia, AN
;
yu, JIN
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells conditoned–medium;
hypoxia-reoxygenation;
cortical neuron;
apoptosis
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of bone marrow mesenchymal stem cells(BMMSCs) conditioned-medium (B-CM) on apoptosis in cultured cortical neurons after hypoxia-reoxygenation in vitro in rats.Methods BMMSCs were isolated,cultured and expanded,the culture me-dium was substituted by new medium that contain no serum when cells grew to 90% confluence.After cultured for 24 hours,the medium was collected as B-CM.Cortical neurons of mouse cultured for 8 days were divided into three groups:normal control groups,hypoxic group,B-CM disposed groups.Cell viability were detected by 3-(4,5-dimethlthiazd-2-yl)-2,5-diphenyltetra-zoliu bromidel (MTT) methods and the apoptosis of neurons was examined by flow cytometry and telemicroscopy on hypoxia for 0,6 hours and hypoxia-reoxygenation for 24 hours,respectively.Results Compared with control group,hypoxia for 6 hours could decrease cell viability,hypoxia-reoxygenation for 24 hours increased the cell apoptosis ratio remarkably(P
