Effects of Early Intervention on Growth Associated Protein-43 and Neuron Cells Apoptosis in Brain of Neonatal Rats
- VernacularTitle:早期干预对新生鼠脑神经生长相关蛋白表达及细胞凋亡的影响
- Author:
yan-hui, CHEN
;
ling, LIU
;
min-rong, CHEN
;
da-guang, CHEN
- Publication Type:Journal Article
- Keywords:
early intervention;
growth associated protein-43;
apoptosis;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of early interventions on growth associated protein-43(GAP-43) and neuron apoptosis in brain of neonatal rats.Methods According to matched-pairs design,30 rats from the same materal rats were divided into two groups:intervention group and control group randomly.The intervention group received the neonatal handling for 14 d and then were kept in an enriched environment for another 14 d.The expression of GAP-43 and the number of neurons apoptosis were detected by immunohistochemistry and TUNEL staining respectively in forehead cortex and hippocampus of rats.The brain functional outcomes of rats were evaluated by water-maze test.Results In prefrontal cortex and hippocampus,the level of GAP-43 immuno-positive response in intervention group was significantly higher than that of control group(P
