Serum-Free Culture and Identification of Neural Stem Cells from Hippocampus of Neonatal Rats
- VernacularTitle:新生大鼠海马神经干细胞的无血清培养及鉴定
- Author:
zi-jin, YANG
;
hai-yan, WANG
;
li-min, WANG
- Publication Type:Journal Article
- Keywords:
neural stem cells;
cell culture;
proliferation;
differentiation
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the best serum-free cultural way in neural stem cells from the hippocampus of the newborn rats and observe the characteristics of growth,proliferation,and induced differentiation of neural stem cells.Methods The neonatal rats′ hippocampus tissues were dissociated mechanically.The cells were cultured in the serum-free cultural medium which were added separately basic fibroblast growth factor(bFGF),epithelium growth factor(EGF),bFGF+EGF by suspended cultural way.The neural stem cells were identified by nestin immunofluorescence.After induced differentiation of embryonic cow serum,the differentiated cells were identified.Results The serum-free medium added bFGF and EGF could perfectly induce neural stem cells to proliferate and the differentiated cells expressed the specific antigen of neurons,astrocytes and oligodendrocytes.Conclusion The serum-free medium with bFGF and EGF can be used to culture neural stem cells from hippocampus tissue of rats in vitro.
