Age-Dependent Feature of Damage of Hippocampus at Different Maturational Stages after Repeated Seizures in Rats
- VernacularTitle:不同成熟期大鼠致后海马结构损伤的年龄依赖性
- Author:
jia-sheng, HU
;
zhi-sheng, LIU
;
ya-ling, HUANG
- Publication Type:Journal Article
- Keywords:
hippocampus;
seizure;
rats;
age-dependent
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe age-dependent feature of damage of hippocampus to different maturational stages rats after kindling repeated seizures.Methods The effects of 5 daily pentylenetetrazol-induced convulsions in different rats beginning at postnatal day 10,20,60(P10,P20,P60)were evaluated.In the 3 groups,Thionin staining method was utilized to observe morphological changes and cell counting of dentate granule cells,CA3,CA1,and hilar neurons.Timm's method of silver sulfide staining was adopted to observe the mossy fiber sprouting.Results 1.Cell counting of CA1,CA3 and hilar neurons in P10 and P20 groups demonstrated no differences from controls in rats,whereas P60 with daily seizures had a significant decrease in CA1,CA3 neurons(8.22?1.88,5.62?1.68 vs 6.31?1.50,3.62?1.40)(t=2.246,2.587 Pa
