Expression of Hypoxia-Inducible Factor-1? in Cultured Cortical Neurons after Hypoxia-Ischemia
- VernacularTitle:缺氧诱导因子-1?在缺氧缺血皮质神经元中的表达
- Author:
li, ZHANG
;
li-hua, LI
;
yi, QU
;
xi-hong, LI
;
chun-lei, YANG
;
meng, MAO
;
de-zhi, MU
- Publication Type:Journal Article
- Keywords:
hypoxia-inducible factor-1?;
neurons;
hypoxia-ischemia
- From:Journal of Applied Clinical Pediatrics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of hypoxia-inducible factor-1?(HIF-1?) protein and its mRNA in cultured cortical neurons after hypoxia,ischemia or hypoxia-ischemia(HI) and explore the possibilities of HIF-1? gene therapy in HI neurons.Methods The in vitro models of HI,pure hypoxia and pure ischemia were established using embryonic day 16-18 rats cortical neurons.Immunohistochemical and in-situ hybridization were performed to examine the expression of HIF-1? protein and its mRNA at different reperfusion time points in neurons.Results The expression of HIF-1? protein was very week in normoxic cultured neurons,but was up-regulated while treated with hypoxia and(or) ischemia.HIF-1? expression reached peak at 4 to 8 h after reperfusion with HI,which were statistically significant higher than that at other time points(Pa=0),and decreased gradually at 12 h.Furthermore,HIF-1? protein expression was significantly higher in HI group compared with that in the pure hypoxia or ischemia group(Pa=0).HIF-1? mRNA reached peak immediately after HI,decreased gradually at 2 h,and returned to the baseline at 8 h after reperfusion.Conclusions HIF-1? expression on cortical neurons is regulated differently with hypoxia,ischemia or HI treatment,HIF-1? gene therapy for HI neurons maybe a useful method in the future studies.
