Expression of Insulin-Like Growth Factor-1 and Its Associated Protein of Mice with Viral Myocarditis
- VernacularTitle:病毒性心肌炎小鼠不同时点胰岛素样生长因子-1及其相关蛋白的表达
- Author:
chun-zhi, HE
;
lin, TIAN
;
rui-zhen, CHEN
;
shuang-jie, LI
- Publication Type:Journal Article
- Keywords:
viral myocarditis;
insulin-like growth factor-1;
insulin-like growth factor-1 receptor;
insulin-like growth factor bin-ding protein-3
- From:Journal of Applied Clinical Pediatrics
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of insulin-like growth factor-1(IGF-1) and its associated protein in Balb/C mice with viral myocarditis.Methods Sixty Balb/C mice were randomly divided into 2 groups:infected group(n=40) and control group(n=20).Mice in infected group were inoculated intraperitoneally with coxsackievirus B3(CVB3) while control mice with 0.1mL of Eagle′s minimal essential medium(EMEM).Blood and myocardial musculature of all mice were collected on the day of 3,7,15 and 30 after inoculation.The expression of IGF-1 and its associated protein in plasma or myocardium were detected respectively by enzyme-labeled immunosorbent assay(ELISA),immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR).Results The expression of IGF-1 and its associated protein of mice with viral myocarditis was higher than those in control group and gradual ascent after mice being infected with virus.The expression reached summit on d15,then decreased on d30,but still sustain in a high level.The difference was significant(F=19.53 P
