Role of Erythropoietin in Relieving Injury of Human Renal Tubular Cell Induced by Postasphyxial-Serum of Neonates
- VernacularTitle:促红细胞生成素减轻窒息新生儿血清诱导人肾小管细胞损伤
- Author:
tao, XIONG
;
wen-bin, DONG
;
ming-yong, WANG
;
cun-liang, DENG
;
kai-gui, XU
- Publication Type:Journal Article
- Keywords:
erythropoietin;
postasphyxial;
serum;
renal tubular epithelial cell;
kidney injury;
infant,newborn
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of erythropoietin(EPO)in relieving the injury of human renal tubular cells (HK-2) induced by postasphyxial-serum of neonates.Methods Human renal proximal tubular cell(HK-2) was used as the target cell.The experiment was designed as control group, asphyxia group,and group of pretreatment with EPO. The attacking concentration of serum was 200 mL/L,then the changes of morphology were observed under inverted microscope,and the cell viability was measured by 3-(4,5-dimethy lthiazcl-2-yl)-2,5-diphenyl-tetazolium bromide(MTT) methods,and the leakage rate of lactate dehydrogenase(LDH) was determined by biochemical methods.Results Compared with control group,the change in morphology of HK-2 was most serious and obvious,and the leakage rate of LDH increased significantly,and the cell viability decreased obviously in asphyxia group.But compared with asphyxia group,the change in morphology of HK-2 was obviously improved,and the leakage rate of LDH decreased and the cell viability increased in group of pretreatment with EPO in a dose-dependent manner except the group of 1 IU/mL.Conclusion EPO can play the role in relieving the injury of renal tubular cells induced by postasphyxial-serum in neonates.
