Expression of Matrix Metalloproteinase-9/Tissue Inhibitor of Metalloproteinase-1 in Rats with Tubulointerstitial Fibrosis
- VernacularTitle:肾小管间质纤维化大鼠基质金属蛋白酶-9/金属蛋白酶组织抑制因子-1的表达
- Author:
yan, WANG
;
de-an, ZHAO
;
da-sheng, YANG
- Publication Type:Journal Article
- Keywords:
tubulointerstitial fibrosis;
matrix metalloproteinase;
tissue inhibitor of metalloproteinases;
rat
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of matrix metalloproteinase-9(MMP-9)/tissue inhibitor of metalloproteinase-1(TIMP-1) in tubulointerstitial fibrosis(TIF) rats and explore the effects of MMP-9 and TIMP-1 in TIF.Methods Forty-eight male Sprague-Dawley(SD) rats were randomly divided into sham-operated group(n=24) and model group(n=24).Results Tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). Histological changes were observed in tubulointerstitium injury under microscope and immunohistochemistry staining was performed to investigate both the expression of MMP-9 and TIMP-1 in TIF by semiquantitative score at d7, d14, d21 after experiment onset.The expressions of TIMP-1 in rats from sham-operated group were lower than those of mo-del group rats at d7, d14, d21 (Pa
