Inhibitive Effect of Interleukin-4,-10 on Cell Apoptosis in Rats with Renal Ischemic-Reperfusion Injury
- VernacularTitle:白细胞介素-4、-10对大鼠肾缺血再灌注损伤细胞凋亡的抑制作用
- Author:
yong, XU
;
shan-lin, CHEN
- Publication Type:Journal Article
- Keywords:
ischemia;
reperfusion injury;
interleukin-4;
interleukin-10;
apoptosis;
rat
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitive effect of interleukin-4(IL-4) and (or) IL-10 on cell apoptosis of renal ischemic-reperfusion injury(IRI) in rats and its mechanism.Methods Thirty female Sprague-Dawley rats were divided into five groups(n=6): sham-operated group,operation+9 g/L NaCl control (NaCl group),operation+IL-4(IL-4 group),operation+IL-10(IL-10 group),operation+ IL-4+IL-10 (IL-4+IL-10 group). The animal model of IRI was set up by clamping bilateral renal pedicles for 45 min.When the clamp was loosed, relevant agents was injected in every group. Apoptosis of renal epithelial cells (TUNEL method) was obserued.Results IL-4 could not reduce level of apoptosis of renal epithelial cells.To some extent,IL-10 could reduce level of renal epithelial cells. The combination of IL-4 and IL-10 was more effective in inhibiting apoptosis of renal epithelial cells.Conclusion The combination of IL-4 and IL-10 is effective in inhibiting apoptosis of renal epithelial cells, indicating that it has a relationship in preventing renal histological and functional damage.
