Expressions of GATA-3 and Th2 Cytokines in Asthmatic Mice and Their Significances
- VernacularTitle:哮喘小鼠肺组织GATA-3及Th2型细胞因子表达的意义
- Author:
li-juan, QIAN
;
li, YANG
;
hai-guo, YU
;
de-yu, ZHAO
- Publication Type:Journal Article
- Keywords:
asthma;
GATA-3;
Th2 cytokines
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions and significances of GATA-3 and Th2 cytokines in asthmatic mice.Methods Sixteen BALB/c mice were randomly divided into control group and asthmatic group.The pathological changes in trachial and pulmonary tissue were observed with HE staining method.The GATA-3 mRNA expression in pulmonary tissue was messured with reverse transcription polymerase chain reaction(RT-PCR).And the IL-5,IL-13 proteins in pulmonary tissue were detected with enzyme linked immunosorbent assay(ELISA).Results HE staining showed infiltration of a great deal of inflammatory cells around the bronchial wall in asthmatic group,while there was no obvious infiltration in control group;Compared with control group,the expressions of GATA-3 mRNA and IL-5,IL-13 protein in pulmonary tissue increased significantly(Pa
