Comparison of Different Methods Used in Detection of Respiratory Syncytial Virus
- VernacularTitle:几种呼吸道合胞病毒检测方法的比较
- Author:
xiao-qi, CHEN
;
ge-lin, XU
;
jia-wu, QUAN
- Publication Type:Journal Article
- Keywords:
respiratory syncytial virus;
infection;
early diagnosis;
immunofluorecence assay;
enzyme linked immunosorbent assay
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select more rapid,sensitive and specific method in detection of respiratory syncytial virus(RSV)directly from clinical specimens.Methods RSV was detected by virus isolation in tissue culture,direct smears and detection by indirect immunofluorecence assay(IFA),rapid culture assay,sandwich enzyme linked immunosorbent assay(ELISA)as well as labbed streptavidin biotin method(LSAB)from 45 specimens(nasopharyngeal aspirates,NPAs) collected from infants and young children with acute lower respiratory tract infection.Results Of 45 NPAs,12 cases(26.7%) were positive by virus isolation,14 cases(31.1%) were positive for RSV by direct detection of RSV antigen by IFA,20 cases(44.4%) were positive with rapid culture assay,4 cases(8.9%)were positive by sandwich ELISA,4 cases(8.9%)were positive by LSAB.Conclusion Rapid culture assay and direct detection of RSV in NPAs direct smears by IFA are rapid,sensitive method in the diagnosis of RSV infections.
