Changes of Malondialdehyde,Myeloperoxidase and Intracellular Free Calcium Contents in Brain Tissue of Fetal Rats Induced by Intrauterine Infection
- VernacularTitle:宫内感染致胎鼠脑损伤组织丙二醛、髓过氧化酶、钙离子的变化
- Author:
xiao-jie, LI
;
wei-liang, LIU
;
wei, PANG
- Publication Type:Journal Article
- Keywords:
intrauterine infection;
fetal brain damage;
malondialdehyde;
peroxidase;
Ca~(2+)
- From:Journal of Applied Clinical Pediatrics
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a model, fetal rat brain damage made by lipopolysaccharide (LPS)-induced intrauterine infection and explore content change and significance of malondialdehyde(MDA),myeloperoxidase(MPO),intracellular free calcium in the development of fetal brain damage.Methods Twenty-eight pregnant rats were injected with LPS(500 ?g/kg)on gestation d20 intraperitoneally in experimental group rats;the same volume of normal saline was injected to 28 rats as control group;there were 8 pregnant rats as blank control rat. Fetal brain water,MDA,MPO,Ca~(2+) contents were measured, fetal brain,placenta for pathological check after injection 2,6,12,24 hours,respectively.Results Brain water,MDA,MPO contents of LPS group were higher than those of controls at 2 h,and Ca~(2+) content of LPS group was higher than that of controls at 6 h,they were continued to be on high level until 24 h.There were histopathological inflammation changes of placenta, edema,inflammation in fetal brain at 6 h ,degeneration and necrosis of nerve cell at 12 h.Conclusions Fetal rat brain damage is made by LPS-induced intrauterine infection.MDA,MPO,Ca~(2+) content increasing in fetal brain tissue that indicates there is lipid peroxidation damage in fetal rat brain damage due to intrauterine infection; damage of free radical,persistent activation of polymorphonuclear,calcium overlord are mechanisms of fetal brain damage .
