Protein Kinase C Regulating Activation on Nuclear Factor-?B in T Lymphocyte in Children with Acute Idiopathic Thrombocytopenic Purpura
- VernacularTitle:蛋白激酶C对急性特发性血小板减少性紫癜患儿T细胞核因子-?B活化的调控作用
- Author:
jin-cheng, XU
;
hong, XIAO
;
chang-lin, WU
;
fang, LIU
- Publication Type:Journal Article
- Keywords:
purpura,thrombocytopenia,idiopathic,acute;
protein kinase C;
nuclear factor-?B
- From:Journal of Applied Clinical Pediatrics
2006;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of protein kinase C(PKC) to regulate the activation of nuclear factor-?B(NF-?B)in T lymphocyte in children with acute idiopathic thrombocytopenic purpura(ITP).Methods Sterility peripheral blood was collected from acute ITP children(n=30)and healthy children(n=30).T lymphocytes were isolated and purified,and divided into 3 groups:control group,PMA group stimulated with PMA,PMA plus H-7 group stimulated with PMA and H-7.The expression of NF-?B and inhibitor protein-?B(I-?B)was detected by immunohistochemical staining and Western blot,respectively.Results The percentage of cells with active NF-?B was significantly higher and the expression level of I-?B was significantly lower in acute ITP PMA group than that in acute ITP control group and normal PMA group,respectively(all P
