Dynamic Changes on Blood S-100 and Protein Expression of Brain in Newborn Rats with Bilirubin Encephalopathy
- VernacularTitle:胆红素脑病仔鼠血清S-100蛋白水平及其脑组织蛋白表达的动态变化
- Author:
li-na, HU
;
zhi-mei, JIANG
;
xiao-jie, LI
;
jing, GAO
- Publication Type:Journal Article
- Keywords:
bilirubin encephalopathy;
nerve tissue protein S-100;
rat,newborn;
immunohistochemitry
- From:Journal of Applied Clinical Pediatrics
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of S-100 protein(S-100) levels in blood in newborn rats with bilirubin encephalopathy.Methods The model was established by administered bilirubin intraperitoneally(200 mg/kg) in newborn rats.The blood and brains samples were taken from 8 rats at the sequential time points of 6,12,24,48,72 and 96 h after the model established.Eight normal rats were used as the control.The dynamic changes of S-100 were detected by enzyme linked immunosorbent assay(ELISA) and immunohistochemical technique.Results S-100 significantly increased in blood of bilirubin encephalopathy newborn rats compared with controls(P
