Protective Effects of Insulin on Acute Global Cerebral Ischemia Reperfusion Injury in Rats
- VernacularTitle:胰岛素注射液对大鼠急性全脑缺血再灌注损伤的保护作用
- Author:
li-li, YU
;
yu-min, CHEN
;
chang-bai, BI
;
li-jin, XU
;
gui-xia, WANG
- Publication Type:Journal Article
- Keywords:
cerebral ischemia reperfusion injury;
rat;
insulin;
neuron specific enolase;
nitric oxide
- From:Journal of Applied Clinical Pediatrics
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects and mechanisms of insulin on reperfusion injury after cerebral ischemia.Methods Sixty-six male Wistar rats were used in this study.All rats were divided into 3 groups as treated group(A),control group(B) and random sham-operated group(C).Four-vessel occlusion was used to establish global cerebral ischemia reperfusion model in study groups.The treated group were divided into 5 groups(A1-A5) and intraperineally injected with biosynthetic human insulin 2 IU/(kg?d) and 50%glucose 2 g/(kg?d) for 7 days,the blood glucose was monitored in preoperative and postoperative 3,6,12,24 h,and the blood glucose was maintained between 3.5-6.5 mmol/L.These animals of control group were given with saline 2 mL/(kg?d) for 7 days in abdominal cavity.All the rats were killed in the seventh day,brain homogenate was collected for detection of neuron specific enolase(NSE)and nitric oxide(NO).The hippocampus was separated for observation of electronic microscope.Results Concentration of NSE in brain tissue in group C was significantly higher than that of group A and group B,while the level in group A was higher than that of group B.Concentration of NO in group C was lower than that of group A and group B while the level of NO in group A was significantly lower than that of group B.Electron microscope showed that the ultrastructure of sham-operated group was nearly normal,damage degree of hippocampal neuron and gliacyte and capillary was gradually worse from group A1,A2 to A4,the damage degree of group B1,B2 and B4 was serious and there was no difference among them.Conclusion Insulin can really promote recovery of the cerebral injury after ischemia reperfusion.
