Ischemic Tolerance and Expression of Hypoxia Inducible Factor-1? in PC12 Cells Induced by Acanthopannx Senticosus Saponins
- VernacularTitle:刺五加皂甙诱导PC12细胞对缺血的耐受与缺氧诱导因子-1?的表达
- Author:
jian, CHEN
;
li, ZHU
;
yong-jin, PAN
- Publication Type:Journal Article
- Keywords:
acanthopannx senticosus saponins;
PC12 cell;
ischemic tolerance;
hypoxia inducible
- From:Journal of Applied Clinical Pediatrics
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study ischemic tolerance induced by acanthopannx senticosus saponins(ASS) on ischemia in PC12 cells and involve expression of hypoxia inducible factor-1?(HIF-1?).Methods An ischemic model was developed in PC12 cell line with treatment of oxygen glucose deprivation(OGD).The protective effects of ASS pretreatment on ischemic tolerance of PC12 cells and whether protective effects of ASS could be inhibited by LY294002(PI3K inhibitor) were analyzed through MTT assay.The induction of phospho-glycogen synthesis kinase-3?(p-GSK-3?) and HIF-1? after pretreatment of ASS and possible blocking effects of LY294002 were detected by Western-blot.Results MTT results showed that after 9 h ischemia,the viability of PC12 cells decreased dramatically(P
