Value of Heparin Blocking Attachment of Respiratory Syncytial Virus to Human Airway Epithelial Cells
- VernacularTitle:肝素阻断呼吸道合胞病毒与呼吸道上皮细胞黏附作用
- Author:
xiao-dong, ZHAO
;
xi-qiang, YANG
- Publication Type:Journal Article
- Keywords:
respiratory syncytial virus;
airway epithelial cell;
attachment;
cellular entry;
glycosaminoglycan
- From:Journal of Applied Clinical Pediatrics
2006;0(22):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of attachment of respiratory syncytial virus(RSV) to human airway epithelial cells.Methods Attachment of RSV to CFBE was quantitatively assessed by flow cytometry.Various polyclonal and monoclonal antibodies to RSV and heparin were pre-incubated with RSV.The blocking effects of these antibodies and heparin on attachment were evaluated.Results CFBE cells reduced capacity being bound by RSV.All the antibodies used were failed to block attachment of RSV on CFBE,whereas heparin blocked RSV attachment in a dose-response manner and the blokade by heparin was almost complete at the concentration of 0.4 mg/L.Conclusions Flow cytometry provides direct evaluation of attachment without growth of virus.Heparin-like molecules on cell surface of CFBE appears to be involved in attachment between RSV and human epithelial cells.
