Analysis of Differentially Expressed Genes between Chronic Hepatitis B and Asymptomatic Hepatitis B Virus Carrying in Children
- VernacularTitle:慢性乙型肝炎和乙型肝炎病毒携带儿童差异表达基因分析
- Author:
cui-lan, TANG
;
zhou, ZHOU
;
zhi, CHEN
;
cheng-ji, LU
- Publication Type:Journal Article
- Keywords:
chronic hepatitis B;
asymptomatic hepatitis B virus carriers;
beta-2-microglobulin;
suppression subtractive hybridization
- From:Journal of Applied Clinical Pediatrics
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the difference of pathogenesis between chronic hepatitis B (CHB) and asymptomatic hepatitis B virus(HBV) carriers(ASCs) in children.Methods Subtracted libraries was constructed by suppression subtractive hybridization (SSH) from peripheral blood mononuclear cell (PBMC) of two groups, beta-2-microglobulin (?_2-MG) was screened as one of differentially expressed genes. Serum was collected from children with CHB and ASCs, the differential expression of ?_2-MG was confirmed by enzyme immunoassay.Results Subtractive libraries were constructed successfully, the differentially expression of ?_2-MG and lactroferrin were up-regulated in CHB children.And the differentially expression of ?_2-MG on the level of protein was confirmed.Conclusion The up-regulation of ?_2-MG in CHB children is involved in the pathogenesis mechanisms.
