Protective effect of astaxanthin on human retinal pigment epithelial cells injured by hydrogen peroxide
10.3980/j.issn.1672-5123.2015.7.07
- VernacularTitle:虾青素对人视网膜色素上皮细胞氧化损伤的保护作用
- Author:
Hai-Rong, ZHUANG
;
Ping, LIU
;
Xue-Zheng, HU
- Publication Type:Journal Article
- Keywords:
astaxanthin;
ultraviolet;
retinal pigment epithelial cells;
apoptosis
- From:
International Eye Science
2015;(7):1148-1150
- CountryChina
- Language:Chinese
-
Abstract:
AlM:To investigate the protective effect of astaxanthin (AST) on human retinal pigment epithelial (RPE) cells against oxidative damage induced by hydrogen peroxide (H2O2).METHODS:Human RPE cells were subcultured, cell activity was detected by MTT, rate of apoptosis was detected by flow cytometry and cell ultrastructure changes were observed under transmission electron microscope. RESULTS: MTT results showed that cell activity elevated to ( 53. 66%± 3. 25% and 70. 43%± 2. 38% after 10-8 mol/L and 10-4 mol/L AST treated. The difference had statistically significant (P<0. 05) compared with oxidative injury group (38. 76%± 3. 74%). Flow cytometry results showed that the apoptosis rate of RPE cells decreased to 30. 23%± 1. 91% and 12. 58%± 2. 12% in AST pretreated group, the difference was significant (P<0. 05) compared with oxidative injury group ( 42. 50%± 1. 94%); Electron microscopy showed that the morphology of cells gradually improved accompanied with the concentration of AST elevated.CONCLUSlON:AST may inhibit hydrogen peroxide-induced apoptosis of RPE cells, it can provide reliable evidence for pursue effective medicine to prevent and treat retina injury.
