Influence of Losartan on Transforming Growth Factor-?1 and Connective Tissue Growth Factor in Rats with Diabetic Nephropathy
- VernacularTitle:氯沙坦片对糖尿病肾病大鼠转化生长因子-?1及结缔组织生长因子的影响
- Author:
jian, YU
;
min-shu, ZOU
;
xue-mei, LIU
;
guo-ming, NIE
;
jing, LIU
- Publication Type:Journal Article
- Keywords:
losartan;
transforming growth factor-?1;
connective tissue growth factor;
diabetic nephropathy
- From:Journal of Applied Clinical Pediatrics
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expression of transforming growth factor-?1(TGF-?1)and connective tissue growth factor(CTGF) protein in renal tissues,and detect the levels of urinary TGF-?1 and CTGF in rats with diabetic nephropathy(DN).To observe the influence of losartan on expression of the two protein in renal tissue and excretion in urine.Methods Wistar rats were treated by intravenous injection of streptozotocin after right nephrectomy to induce DN rat model.The DN rats were randomly divided into two groups:DN experimental group and losartan treated group.The expression of TGF-?1 and CTGF in renal tissue were determined with immunohistochemical staining,urinary TGF-?1 and CTGF were assayed by enzyme-linked immunosorbent assay(ELISA) at 6,12 weeks respectively.Results Compared with losartan treated group,urinary protein excretion and the protein expression of TGF-?1 and CTGF significantly increased(P
