Protective effect ofα-mangostin on retinal light damage in mice
10.3980/j.issn.1672-5123.2015.7.06
- VernacularTitle:α-倒捻子素对小鼠视网膜光损伤的保护作用
- Author:
Yuan, FANG
;
Tu, SU
;
Ping, XIE
;
Song-Tao, YUAN
;
Wen, FAN
;
Yi-Dan, XU
;
Zi-Zhong, HU
;
Qing-Huai, LIU
- Publication Type:Journal Article
- Keywords:
retinal light damage;
α - mangostin;
oxidative stress;
electroretinogram;
outer nuclear layer;
malondialdhyde
- From:
International Eye Science
2015;(7):1143-1147
- CountryChina
- Language:Chinese
-
Abstract:
AlM:To discuss the protective effect ofα-mangostin on retinal light damage in mice.METHODS:Totally 30 Balb/c mice, aged 6~8wk, were randomly divided into the control group, light-exposure group and α-mangostin group. Every group contained 10 mice. Mice of α-mangostin group were treated with alpha-mangostin at the dose of 30mg/( kg · d ) body weight by intragastric administration daily for 7d, and then exposed to white light at the 5th d. The light-exposure group and α-mangostin group were exposed to 5 000 ± 200lx white light-emmiting diodes (LEDs) for continuously 1h to establish the mice model of retinal light damage. Flash -electroretinograme was recorded 72h after light exposure. The changes in retinal morphology of mice were observed by light microscopy. Retinas were extracted to detect the malondialdhyde ( MDA ) content change of the retinal homogenate.RESULTS: Flash-electroretinogram ( F-ERG ) showed that retinal dysfunction was less severe in α-mangostin group than in light-exposure group ( P<0. 05 ). Light microscopy test showed that retina structural damage was less severe in α-mangostin group than in light-exposure group (P<0. 05). The level of MDA in retinal tissue of α-mangostin group was significantly lower when compared with light-exposure group (P<0. 05).CONCLUSlON: α-mangostin inhibits lipid peroxidation induced by light damage and protect retina against light damage.
