Experimental Study on Activation of Caspase-3 and Apoptosis of K562 Cell Induced by Iron-Deprivation
- VernacularTitle:铁剥夺诱导K562细胞凋亡与Caspase-3激活的实验研究
- Author:
you-cai, TANG
;
guo-cun, JIA
;
feng-yi, LI
;
qing-kui, LIAO
;
bin, CHEN
;
wen-zhong, NIU
- Publication Type:Journal Article
- Keywords:
iron-deprivation;
K562 cell;
Caspase-3;
apoptosis
- From:Journal of Applied Clinical Pediatrics
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the relationship between apoptosis of K562 cell induced by iron-deprivation and activation of Caspase-3.Methods K562 cells were treated with desferrioxamine(DFO) in different dosages were collected at different time points.K562 cells were labelled with Annexin V/PI,and then the rate of apoptosis was measured by flow cytometry;The activation of Caspase-3 were detected by colorimetric method with pAN labelled substrate;The active protein of Caspase-3 were analyzed by Western blot.Results When K562 cells were treated with different concentrations of DFO,the apoptosis rate and the activity of Caspase-3 increases gradually.When K562 cells were incubated with DFO(50 ?mol/L and 100 ?mol/L) 24 h later,the enzymatic activity of Caspase-3 increases dramatically more than that of control group,and the difference was significantly(P0.05).All those effect above can be counteracted by equal mole concentration of FeCl_3.Conclusion Iron-deprivation maybe induce the apoptosis of K562 cell by chelating intracellular iron and activing Caspase-3.
