Influence of Iron on Mitochondrion Membrane Potential and Apoptosis of Human Leukemia-60 Cells
- VernacularTitle:铁对白血病细胞HL-60线粒体膜电位及凋亡的影响
- Author:
qiu-tang, ZHANG
;
dao, WANG
;
yu-feng, LIU
- Publication Type:Journal Article
- Keywords:
deferoxamine;
human leukemia-60 cell;
apoptosis;
mitochondrion membrane potential
- From:Journal of Applied Clinical Pediatrics
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of mitochondrion membrane potential(??m) in apoptosis induced by an iron chelator(deferoxamine,DFO),and to explore the mechanism of this apoptosis in human leukemia-60(HL-60) cells.Methods HL-60 cells were co-cultivated with various concentration of DFO and FeCl_3 for certain time,resultingin status of intracellular iron deprivation and rich iron.Cell vital force was determinded by the MTT method.The cells were examined by phase contrast microscopy,flow cytometry(FCM) for evidence of apoptosis,and also by FCM for ??m.The transcription of the apoptogene of bax was detected by hybridization in situ.Results The cell growth rate assumed descent tendency.DFO could induce the apoptosis and descent ??m of HL-60 cells(P
