Effect of Glutamine and Epidermal Growth Factor on Neonatal Rats with Necrotizing Enterocolitis
- VernacularTitle:谷氨酰胺和表皮生长因子对新生鼠坏死性小肠结肠炎的影响
- Author:
lin, YANG
;
yu-ning, LI
- Publication Type:Journal Article
- Keywords:
glutamine;
epidermal growth factor;
necrotizing enterocolitis;
neonatal rat;
intestinal mucosa
- From:Journal of Applied Clinical Pediatrics
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of glutamine(Gln) and epidermal growth factor(EGF) on small intestinal repair in neonatal rats with necrotizing enterocolitis(NEC).Methods Forty neonatal rats of NEC model were divided into 5 groups:group A(control group),group B(NEC model),group C(NEC+Gln),group D(NEC+EGF+Gln).After NEC model of neonatal rats,all the neonatal rats were returned to their mothers.In the 4~(th) day,all the subjects were put to the death.Intestinal tissue located at the boundary of ileum and cecum was obtained to observe histological changes.Morphological changes were measured under a light microscope,and expression of proliferating cell nuclear antigen(PCNA) as an index of cell proliferation was observed using immunohistochemical staining and image analysis,expression of apoptosis was observed by TUNEL staining and image analysis.Results In group B,the histological lesions ranged was different,and was graded 3 score.In group C,D,the histological lesions ranged was graded 1 score.The number of(PCNA) in group B was lower than that in group A(P
