Significance of Changes of Endogenous Hydrogen Sulfide in Rats with Doxorubicin-Induced Heart Failure
- VernacularTitle:多柔比星致心力衰竭大鼠血浆及心肌组织内源性硫化氢变化的意义
- Author:
yu-wen, SU
;
jun-bao, DU
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
doxorubicin;
heart failure;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in hydrogen sulfide(H_2S) level in plasma and myocardial tissues of doxorubicin-induced heart failure rats,and to study the role of H_2S in the development of doxorubicin-induced heart failure.Methods Male Wistar rats were randomly divided into 2 groups as follows:the doxorubicin group(n=12),in which 2.5 mg/kg of doxorubicin was injected intraperitoneal once a week for 10 weeks(total dose of 25 mg/kg);the control group(n=9),in which an(equivalent) volume of physiological saline was administered weekly for a total of 10 weeks.The observation of behavior was taken at the same time.Hemodynamic and echocardiographic measurements were obtained 10 weeks after treatment.Meanwhile,H_2S concentrations in serum and myocardial tissues were evaluated by modified sulfide electrode method.The changes of H_2S level between 2 groups were analyzed.Results The rats treated with doxorubicin showed inanimate behavior,decrease of the body temperature,activities and food intake,faster breathing,significant loss of weight,the cumulative mortality was 33%.Left ventricular systolic pressure(LVSP),difference of left ventricular pressure[△LVP=LVSP-left ventricular diastolic pressure(LVDP)] and left ventricular peak rate of contraction(+LV dp/dtmax),left ventricular peak rate of relaxation(-LV dp/dtmax)were significantly reduced in the group of doxorubicin rats(P
