Viral Transactivation of Transcription in Steroid Responsive Simple Nephrotic Syndrome Mediated by Nuclear Factor-Kappa B/Inhibitor Protein-Kappa B Signal Pathway
- VernacularTitle:核因子-?B/抑制蛋白-?B信号通路介导的激素敏感型单纯性肾病患儿病毒基因反式激活
- Author:
yu-hong, TAO
;
zheng, WANG
;
chan-sheng, LI
;
xiao-shi, ZHU
- Publication Type:Journal Article
- Keywords:
steroid responsive simple nephrotic syndrome;
inhibitor protein kappa B;
nuclear factor-kappa B;
viral transactivation of transcription;
respiratory tract viruses
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of nuclear factor-kappa B(NF-?B)/inhibitor protein-kappa B(I?B) signal pathway in viral transactivation of transcription in steroid responsive simple nephrotic syndrome(SRSNS).Methods Children with SRSNS(inclu-ding active stage and remissive stage) were examined,and were compared to children with nephritic nephrosis,secondary glomerular di-seases,bronchiolitis and healthy children.Electro-mobility shift assays,reverse transcription-polymerase chain reaction(RT-PCR) and enzyme-linked immunosorbent assay(ELISA) were used to detect the activity of NF-?B,the gene expression of respiratory tract viruses (including respiratory syncytial virus and influenza virus) in peripheral blood mononuclear cells(PBMCs) and the levels of viral antibody in plasma,respectively.The protein levels of I?B? and IL-8 were measured through Western blot and ELISA in SRSNS at active stage and healthy children.Results Compared with SRSNS at remissive stage and other groups,the activity of NF?B in SRSNS at active stage was much higher.And there was a positive linear correlation trend between the activity of NF-?B and the gene expression of respiratory tract viruses in SRSNS at active stage.With healthy children,the level of IL-8 in plasma from SRSNS at active stage was significantly increased.There was a positive correlation between the activity of NF?B and the level of IL-8(r=0.88 P
