Effect of Postasphyxial-Serum of Neonate in Inducing Injury of Human Renal Tubular Cell
- VernacularTitle:新生儿窒息后血清在诱导人肾小管细胞损伤中的作用
- Author:
min, CAO
;
wen-bin, DONG
;
ming-yong, WANG
;
cun-liang, DENG
;
feng, CHEN
;
kai-gui, XU
- Publication Type:Journal Article
- Keywords:
asphyxia;
serum;
renal injury;
infant,newborn
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of postasphyxial-serum of neonate in inducing injury of human renal proximal tubular cells(HK-2 cells).Methods HK-2 cells were used as target cell.The neonatal different concentration postasphyxial-serum of 1,3,7 days after asphyxia were used as attacking means.The experimental groups were divided into 15 groups:the 2.5%,5.0%,10.0%,(20.0%) attacking concertration groups of 1,3,7 day after asphyxia and control group of each concertration.The culture medium and concertration of the control group and the experimental groups were the same.The changes of morphology were observed under inverted microscope,the cell viability was measured by 3-(4,5-dimethyl-2-thiazoly1)-2,5-diphenyl-2H-tetrazolium bromide(MTT) method and the leakage rate of lactate dehydrogenase(LDH) was determined by biochemical methods.Results Compared with control group,the changes in morphology of HK-2 were most serious and obvious,the cell viability were obviously decreased(all P
