Levels of Interferon ? and Interleukin-10 in Sera and in Culture Supernatants of Peripheral Blood Mononuclear Cells from Children with Chronic Hepatitis B Virus Carriers and Their Clinical Significance
- VernacularTitle:慢性乙型肝炎病毒携带儿童血清及外周血单个核细胞培养上清干扰素?和白细胞介素-10的水平及其意义
- Author:
cui-lan, TANG
;
zhou, ZHOU
- Publication Type:Journal Article
- Keywords:
hepatitis B virus,chronic;
peripheral blood mononuclear cells;
hepatitis B virus core antigen;
interferon ?;
interleukin-10
- From:Journal of Applied Clinical Pediatrics
2006;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the levels of interferon ?(IFN-?) and interleukin-10(IL-10) in sera and in hepatitis B virus(HBV) core antigen(HBcAg) stimulated culture supernatants of peripheral blood mononuclear cells(PBMC) from chronic HBV carriers children and their clinical significance.Methods Five milliliter peripheral blood was collected from 21 HBeAg-positive children(group A),20 HBeAg-negative children(group B) and 19 normal controls(group C),sera and PBMC was isolated,after 72 h culture of PBMC with HBcAg or not.The levels of IFN-? and IL-10 in sera and in HBcAg stimulated or not stimulated culture supernatants of PBMC were detected.Results The level of sera IFN-? had no difference between each group;the sera level of IL-10 in group A and B were significantly higher than that of group C.The level of IFN-? in HBcAg stimulated culture supernatants of PBMC from group B and C were higher than that from group A;while IL-10 levels in HBcAg stimulated culture supernatants from group A and B were higher than that from group C.Conclusions The level of IL-10 and the lower reactivity to HBcAg of PBMC play roles in the mechanisms of chronic HBV carriers children.
