Influence of Pranlukast on Expression of Eotaxin-3 in Bronchial Epithelia Cells
- VernacularTitle:普仑斯特对人支气管上皮细胞表达嗜酸性粒细胞趋化因子-3的影响
- Author:
wei-lin, OU
;
huan, WEI
;
zhi-ying, FENG
;
chun-jiang, ZHU
;
ming-ci, ZHENG
- Publication Type:Journal Article
- Keywords:
bronchial;
epithelial cell;
leukotrienes D4;
interleukin-4;
eotaxin-3;
pranlukast
- From:Journal of Applied Clinical Pediatrics
2006;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether leukotriene D4 (LTD4) regulates eotaxin-3 (Eot-3) expression in bronchial epithelial cells, and study effect of pranlukst on the regulation.Methods BEAS-2B cells and normal human bronchial epithelia cells were pre- treated with LTD4 for 1 hour,stimulated with interleukin-4, the cells were incubated for 24 hours. Eot-3 protein in supernatant were measured by enzyme linked immunosorbent assay(ELISA). The cells were pretreated with pranlukast in different concentration, then the above procedure was repeated. Results The untreated bronchial epithelial cell expressed Eot-3 protein on a very low level. After stimulating with IL-4 and incubating for 24 hours, Eot-3 production increased significantly. Pretreating the cells with LTD4 enhanced the inducing effect of IL-4. Pranlukast inverted the upregulation of LTD4. Conclusions Upregulating the expression of Eot-3 induced by IL-4 on bronchial epithelial cells may explain partially the mechanism of leukotrienes involving airway allergic inflammation of asthma. The invertion impact on upregulation of LTD4 by pranlukast may be one of mechanisms that leukotrienes receptor antagonist cure asthma.
