Influence of Marrow-Derived Mesenchymal Stem Cells on Expression of Intercelluar Adhesion Molecule-1 in Lung of Neonatal Rats Suffered Hyperoxia
- VernacularTitle:骨髓来源间充质干细胞对高氧新生大鼠肺组织细胞间黏附分子1表达的影响
- Author:
zhao-fang, TIAN
;
jiang, DU
;
bin, WANG
;
xiao-yang, HONG
;
zhi-chun, FENG
- Publication Type:Journal Article
- Keywords:
bone marrow;
mesenchymal stem cells;
rat;
hyperoxia;
cell adhension molecules
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of marrow-derived mesenchymal stem cells(MSCs) on intercelluar adhension molecule-1(ICAM-1) in lung of neonatal rats suffered hyperoxia.Methods Marrow-derived MSCs were separated,cultured,amplificated and labeled with 5bromo 2′-deoxy-uridinel(BrdU);after suffered 95% oxygen for 7 days,24 three-day-old SD rats were randomly divided into group A,B and C,and they were injected intraperitoneally with MSCs of 1?10~4,5?10~4 PBS,respectively.Seven days later,immunocytochemisty was used to determine the expression of BrdU and ICAM-1,and value of radical alveolar counts(RAC) of lungs were counted for histopathological study under light microscope.Results Both group A and B,the labeled MSCs had been(detec)-ted in lungs,and there existed significant variance between two groups(P
