Application of Different Experimental Methods for Determining Neural Stem Cells in Hippocampus from Human Fetal Brain
- VernacularTitle:不同实验方法在人胎脑海马神经干细胞检测中的应用
- Author:
xiao-juan, YIN
;
zhi-chun, FENG
- Publication Type:Journal Article
- Keywords:
neural stem cell;
brain;
hippocampus
- From:Journal of Applied Clinical Pediatrics
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a precise and dependable method for determining neural stem cells(NSCs)in hippocampus from human fetal brain.Methods Ten cases of embryo at gestational age 32 weeks and induction of labor with water bag were collected,affused and sliced including frost slice and paraffin slice for determining alteration of tissue and NSCs in hippocampus from human fetal brain with HE stain and immunohistochemical staining under light microscope.Results Nestin proteins located in NSCs and the number of NSCs were less in paraffin slice of tissue than those in frost slice.Integrality of structure of tissues and cells in paraffin slice excelled in frost slice.Conclusions There is a precise and dependable method including induction of labor with water bag and allusion and frost slice,which is necessary for wide useness in study of NSCs in human fetal brain.
