Effect of Myriocin on Hypertrophy of Glomerular Mesangial Cells and Extracellular Matrix Production Induced by High Glucose
- VernacularTitle:多球壳菌素对高糖诱导肾小球系膜细胞肥大及细胞外基质合成的影响
- Author:
zhao-hua, XIAO
;
jian-hua, ZHOU
;
heng-sheng, WU
- Publication Type:Journal Article
- Keywords:
myriocin;
glomerular mesangial cell;
glucose;
extracellular matrix;
cellular hypertrophy
- From:Journal of Applied Clinical Pediatrics
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of extracellular matrix(ECM) production and hypertrophy of glomerular mesangial cells(GMCs) induced by high glucose(HG),and the inhibitory role of myriocin(ISP-1).Methods GMC cultured with normal glucose(5.6 mmol/L D-Glucose,NG),HG(25.2 mmol/L D-Glucose) and HG plus ISP-1(100 mg/L) for different durations(0,24,48,(72 h)).The sizes of GMC were indicated by forward scatter intensity,measured by flow cytometery,and the levels of fibronection(FN),collagen Ⅳ(Col Ⅳ),laminin(LN),precollagen Ⅲ(Pcol Ⅲ) and hyaluronic acid(HA) in the supernatant of cultured GMC were detec-(ted) by ELISA.Results Compared with NG,HG could induce GMC hypertrophy(P
