Effects of Fosinopril on Transforming Growth Factor-Beta 1 Secretion and mRNA Expression of Cultured Rats Glomerular Mesangial Cell
- VernacularTitle:福辛普利对大鼠肾小球系膜细胞转化生长因子-?_1蛋白及其mRNA表达的影响
- Author:
li-na, WANG
;
zhi-hong, HAO
;
zhi-yuan, WENG
;
li, YU
;
you-xiang, ZHANG
- Publication Type:Journal Article
- Keywords:
angiotensin-converting enzyme inhibitor;
fosinopril;
mesangial cell;
transforming growth factor-beta_1
- From:Journal of Applied Clinical Pediatrics
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of fosinopril(FOS),a new generation angiotensin-converting enzyme inhibitor(ACEI),on protein and mRNA expression of transforming growth factor-?_1(TGF-?_1) of rat glomerular mesangial cell(GMC) induced by lipopolysaccharide(LPS);to demonstrate the preventive mechanism against glomerular sclerosis by applying FOS.Methods The cultured GMC in classic way were divided into 3 groups:control group;LPS group;LPS+FOS group.TGF-?_1 concentration in GMC supernatant fluid was detected by ELISA;TGF-?_1 mRNA expression was determined by semiquantitative real-time RT-PCR.Results LPS group was obviously higher than control groups in TGF-?_1 secretion and mRNA expression,while LPS+FOS group decreased distinctively in TGF-?_1 secretion and mRNA expression compared with LPS group.Conclusions FOS has obviously inhibited on TGF-?_1 expression of rat GMC both at protein level and mRNA level,which reveals that it may be an important mechanism by FOS on restraining the development of glomerulosclerosis.
