Effect of Intrauterine Infection and Interuterine Asphyxia to Fetal Rats′ Brain Damage,Cell Apoptosis and Expression of Glial Fibrillary Acidic Protein
- VernacularTitle:宫内感染和窒息联合作用对胎鼠脑损伤及细胞凋亡和胶质纤维酸性蛋白表达的影响
- Author:
xiu-yong, CHENG
;
hui-fang, DONG
;
chang-lian, ZHU
- Publication Type:Journal Article
- Keywords:
infection;
asphyxia;
brain damage;
apoptosis;
glial fibrillary acidic protein;
rat
- From:Journal of Applied Clinical Pediatrics
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out the associated effect of intrauterine infection and interuterine asphyxia to fetal rat′s brain damage,cell apoptosis,and expression of glial fibrillary acidic protein(GFAP).Methods Pregnant rats of gestation 18 days were randomly divided into four groups:1.NS plus sham operation,2.intrauterine infection,3.intrauterine asphyxia,4.intrauterine infection plus intrauterine asphyxia.The fetal rats′ brains were taken out 72 h after different disposal and given HE coloration,immunohistochemistry of TUNEL and GFAP,respectively.Results The level of brain cell edema and tissue disorganization of group intrauterine infection plus intrauterine asphyxia were more serious than those of group intrauterine infection or group intrauterine asphyxia.TUNEL and GFAP had the same results:The number of positive cells in group intrauterine infection plus intrauterine asphyxia more than that in group intrauterine infection,and which in group intrauterine asphyxia more than that in group NS plus sham operation.There was significant difference between the first three groups and the group NS plus sham operation(P=0).There was also significant difference between group intrauterine infection plus intrauterine asphyxia and group intrauterine infection or group intrauterine asphyxia(P=0).Conclusions Both intrauterine infection and intrauterine asphyxia may induce premature rat brain damage,the association of intrauterine infection and intrauterine asphyxia may aggravate the degree of fetal rat brain damage,also increase the number of apoptosis cell and the expression of GFAP.
