Role of Retinoic Acid Receptor Antagonist on Immunoglobulin M Synthesis by Cord Blood Lymphocytes
- VernacularTitle:视黄酸受体拮抗剂对脐血淋巴细胞免疫球蛋白M生成的作用
- Author:
bing-bing, WU
;
wei-ping, WANG
;
yi, YANG
- Publication Type:Journal Article
- Keywords:
retinoic acid;
retinoic acid receptor;
cord blood;
lymphocytes;
immunoglobulin M
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Retinoic acid receptor(RAR?) selective antagonist,Ro41-5253(Ro) was used in the counteract test,to confirm the role of RAR? in enhancement of IgM synthesis in cord blood lymphocytes(CBLs) by retinoic acid(RA).Methods CBLs were cultured in vitro and stimulated with or without RAR? agonist RA and(or) antagonist Ro.The cells were harvested at 24 hours and 48 hours of culture time to measure the levels of gene expression of RARa,IL-4 and IL-6.ELISA was used to measure the concentration of IgM in the supernatant of 5 days culture.Results Ro could inhibit the promotion of RA on IgM synthesis in CBLs.RA could up-(regulate) RAR? gene expression,which could be restrained by Ro.Ro also could counteract the up-regulation of RA on IL-4 and IL-6 gene expression.Conclusion The effect of RA on IgM synthesis in CBLs is modulated by RAR?.
