Expressions of Interferon Gamma and Transforming Growth Factor Bata1 in Hyperoxia-Induced Lung Injury and Their Significances
- VernacularTitle:?干扰素和转化生长因子?_1在高氧肺损伤中的表达及其意义
- Author:
yan-yan, WU
;
zhong-yi, LU
;
feng, XU
- Publication Type:Journal Article
- Keywords:
hyperoxia;
interferon gamma;
transforming growth factor bata1
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of interferon gamma(IFN-?) and transforming growth factor bata1(TGF-?_1) in animal model of hyperoxia-induced lung injury,and to explore the mechanism of fibrosis.Methods Thirty-two juvenile Wistar rats were randomly divided into room-air group and hyperoxia group,the hyperoxia group were further divided into 3 subgroups of hyperoxia-exposure for 3,7 and 14 days.The mRNA levels of IFN-? and TGF-?_1 in the lung were measured by reverse-transcription polymerase chain reaction(RT-PCR).The expressions of IFN-? and TGF-?_1 protein were measured by immunohistochemical(stai)-ning.Results The level of IFN-? mRNA of groups under hyperxia-exposure for 3,7 and 14 days were higher than that of control group.The expression of IFN-? mRNA notably elevated and was up to its peak(P
