Changes of N-Methyl-D-Aspartate-Receptor-Channels Current in Developing Hippocampal Neurons after Hypoxia and Effect of Adenosine Intervention
- VernacularTitle:发育中的海马神经元缺氧后N-甲基-D-门冬氨酸受体通道电流变化及腺苷干预作用
- Author:
zhen-biao, LI
;
xi-ru, WU
;
yu-wu, JIANG
- Publication Type:Journal Article
- Keywords:
whole-cell recording;
hippocampal;
neuron;
development;
N-methyl-D-aspartate-receptor-channels;
hypoxia
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of N-methyl-D-aspartate(NMDA)-receptor-channels current in developing hippocampal neurones during hypoxia and effect of adenosine intervention.Methods Whole-cell patch-clamp techniques cultured hippocampal neurons whose cultured day were 6 days and 16 days respectively,the amplitude of the NMDA-receptor-channels currents of hippocampal neuron were determined.And the effect of hypoxia on the NMDA-receptor-channels current,and adenosine regulatory mechanisms in cultured hippocampal neurons were explored.Results During hypoxia,compared with control group,the amplitude of the NMDA-receptorchannels currents of hippocampal neuron whose cultured day was 6 days were significantly increased(P
