Correlation between Mitochondrial Membrane Potential and Topiramate′s Neuroprotection
- VernacularTitle:线粒体膜电位与托吡酯神经保护作用的关系
- Author:
xin-min, LIU
;
da-bin, WANG
;
dian-ding, ZOU
;
meng, MING
- Publication Type:Journal Article
- Keywords:
topiramate;
hippocampal neuron;
mitochondrial membrance potential;
glutamate;
neuroprotection
- From:Journal of Applied Clinical Pediatrics
2004;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study topiramate′s neuroprotection on primary cultured hippocampal neurons which were injuried by glutamate and its mechanism.Methods The primary cultured hippocampal neurons were made as the research object,and excitotoxicity model was exected with glutamate.Hippocampal neuron survival was assessed by MTT method and hippocampal neuron mitochondrial membrance potential(MMP) was evaluated by fluorescence microscope and flow cytometry.Results Hippocampal neuron survival of normal control was(98.4?0.8)%,and the survival of glutamate model group was(59.6?3.2)%,at the same time,two topiramate′s groups′ cell survivals was(74.1?0.5)% and(79.2?3.4)%,and topiramate with two levels all could obviously increase hippocampal neuron survival((P
