Changes of Ultrastructure of Xenograft Model of Human Hemangioma on Nude Mice
- VernacularTitle:人血管瘤裸小鼠移植模型的超微结构变化
- Author:
yun-man, TANG
;
wen-ying, LIU
;
song, YU
;
qiang, PENG
;
yong, WANG
- Publication Type:Journal Article
- Keywords:
hemangioma;
models,animal;
mice,nude;
endothelial cells;
ultrastructure
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the development of ultrastructure in grafts from a xenograft model of human hemangioma on nude mice.Methods Human infantile hemangioma tissue was obtained in surgery and planted into subcutaneous layer of nude mice in small pieces.The grafts were harvested on the 2~(nd),5~(th),15~(th),30~(th),45~(th) day and then at 2~(nd),3~(rd),4~(th),6~(th) month for transmission electron microscopic view.Results In early stage after transplantation,signs of degeneration and necrosis were more evident.On the 30~(th) day,the number of endothelial cells increased and some of the endothelial cells were larger in size with high nuclear to plasm ratio.At the 2~(nd) month,the number of endothelial cells increased,while some of these cells turned flat.Later,high endothelial cells decreased in number and collapse and pyknosis of plasm,formation of secondary lysosome,margination of chromatin,fragmentation of nucleus were evident with surrounding deposition of adipose and collagen.Conclusions With xenotransplantation of human infantile hemangioma tissue onto nude mice,the grafts may survive ischemia and proliferate,and then gradually involute.The development resembles a natural course of human infantile hemangioma.
