Polymerase Chain Reaction-Sequence Special Primers in Detecting Gene Polymorphism of Pediatrics Diseases
- VernacularTitle:聚合酶链反应-序列特异性引物方法检测儿科疾病基因多态性
- Author:
rui, YANG
;
xian-jun, YANG
;
chuan, QIN
;
jun, LIU
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
sequence specific primer;
polymorphism,single nucleotide
- From:Journal of Applied Clinical Pediatrics
2004;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an approach of polymerase chain reaction-sequence special primers(PCR-SSP) to perform polymorphism analysis for multiple genes simultaneously,in order to be used in clinical analysis.Methods The optimized PCR-SSP approach was used to analyze the polymorphism of the following genes:mutation of-308A/G and-238G/A in tumor necrosis factor-?((TNF-?)),-174G/C in interleukin-6(IL-6).Results Polymorphism analysis of multiple genes and many clinical samples could be simultaneously performed with one PCR program,showing clear genotype,quick and accurate genotype.Conclusion The optimized(PCR-SSP) is suitable for polymorphism analysis of the large-sample polygenic mononcleeotide point mutation,and it can be widely applied in clinical test for low cost,quickness and accuracy.
