Influences of Human Cytomegalovirus on Proliferation of Lymphocyte Progenitor and Its Interference Methods
- VernacularTitle:巨细胞病毒感染对淋巴系祖细胞增殖的影响及其干预措施
- Author:
hong-ying, LI
;
wen-jun, LIU
;
qu-lian, GUO
;
zheng-hua, DENG
;
jiang, LIN
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus;
colony-forming units,lymphocyte;
astragalus;
ganciclovir
- From:Journal of Applied Clinical Pediatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of human cytomegalovirus (HCMV) on proliferation of colony forming unit T-lymphocyte (CFU -TL)and its interference methods. Methods Normal CFU - TL culture was used as blank control. Normal CFU- TL culture system plus inactivated HCMV fluid as inactivated HCMV control. The dilution of 1:10,1:100,1:1000 were added into CFU -TL colonies culture system directly as infected group. Astragalus (AMI) and ganciclovir(GCV) were added into culture system with HCMV dilution of 1:10 as experimental group. By methylcellulose semi-solid culture, different concentrations of HCMV - AD1699 affect CFU-TL and interfered by astragalus AMI, GCV. CFU - TL were surveyed. The effect of HCMV on CFU-TL proliferation was measured by MTT; HCMV-AD169 DNA in CFU-TL was found by PCR. Results 1. Compared with control group, the numbers of CFU -TL in the HCMV infection groups decreased significantly(P
