Detection of Ampicillin - Resistant Genes and Studies on the Molecular Mechanisms of Ampicillin - Resistant Haemophilus Influenzae
- VernacularTitle:流感嗜血杆菌耐药基因及耐药机制分析
- Author:
tian-ying, ZHONG
;
hui-yun, WANG
;
hua, TAN
;
qian, CHEN
;
zheng, HU
;
zu-huang, MI
;
fu-li, CHI
- Publication Type:Journal Article
- Keywords:
child;
haemophilus inf luenzae;
bata-lactamase;
gene
- From:Journal of Applied Clinical Pediatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanisms of ampicillin- resistant haemophilius influenzae (Hi)in Nanjing. Methods One hundred and fifty- eight strains of Hi isolated from children were collected to detect bata-lactamase. TEM and ROB bata- lacta-mase genes were detected by polymerase chain reaction (PCR) ,and cloned into T vector for sequencing. Results The rate of ampicillin resistance was 41. 77% in Hi isolated from children in Nanjing,40.51 % was found to be bata-lactamase production. Eighty-nine strain were TEM positives, 1 strain was ROB positive,63 strains bata - lactamase positive ampicillin- resistant Hi were identified. The resistance mechanism of ampicillin resistant Hi was production of bata - lactamase , mainly TEM - type enzyme. Two bata - lactamase negative ampicillin - resistant Hi were identified , predicts the other mechanisms of ampicillin - resistant Hi was occuered yet . One strain of non -TEM - type,and non - ROB - type bata - lactamase - producing Hi was identified. Conclusions Ampicillin - resisitant in Hi isolated from children in this region is challenging. TEM bata - lactamase is the principal mechanism of ampicillin - resistant of Hi.
