Effect of Oleic Acid on Differentiation of SW872 Preadipocytes
- VernacularTitle:油酸诱导SW872前脂肪细胞分化的作用
- Author:
cheng-zhi, YE
;
long-jiang, ZHANG
;
hong-wei, WANG
;
han-hua, LIN
- Publication Type:Journal Article
- Keywords:
oleic acid;
SW872 preadipocytes;
differentiation;
triglyceride;
transcription factor
- From:Journal of Applied Clinical Pediatrics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of oleic acid on the differentiation of SW872 preadipocytes.Methods SW872 prea-(dipocytes) were cultured and induced to differentiate by 0.6 mmol/L oleic acid in vitro.After 24 h,48 h and 72 h of differentiation,the morphological changes of SW872 preadipocytes were observed and the differentiation rate was assayed by oil-red O staining.In addition,triglyceride(TG) mass was detected by chemical colorimetry methods.During the differentiation of SW872 preadipocytes,transcription factors including peroxisome proliferator activated receptor-?_2(PPAR-?_2) and CAAT/enhancer binding protein-?(C/EBP-?)(mRNA) were also measured by reverse transcription-polymerase chain reaction(RT-PCR).Results 1.SW872 preadipocytes were fibroblastic and had no obvious fat droplet in cytoplasm.However,when stimulated for 72 hby 0.6 mmol/L oleic acid,SW872 preadipocytes became more bigger and rounder and differentiated into mature adipocytes with lots of fat droplets in the cells.2.Compared with that of predifferentiation,the concentration of TG mass increased by 14 folds after 72-hour differentiation(P
