Influence of Tumor Necrosis Factor-? on Blood Brain Barrier Permeability and Its Mechanism
- VernacularTitle:肿瘤坏死因子-?对体外血脑脊液屏障模型通透性的影响及其机制研究
- Author:
fei, YIN
;
wei-min, ZENG
;
jing, PENG
;
na, GAN
;
hong-yuan, ZHANG
- Publication Type:Journal Article
- Keywords:
blood brain barrier;
permeability;
tumor necrosis factor-?
- From:Journal of Applied Clinical Pediatrics
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the changes and possible mechanism of the blood brain barrier(BBB) permeability induced by tumor necrosis factor(TNF-?) in vitro.Methods BBB model was established by coculturing allogenic brain microvessel end othelial cell(BMEC) and astrocyte(AS).The BBB model was divided randomly into normal control group,TNF-? group and Y-27632 pretreatment group.The changes of BBB permeability were evaluated by Gamma radioim muno assay counter.Results The Gamma radioimmuno assay indicated that the marker,~(125)I-BSA,across the BBB model in vitro was significantly increased after TNF-? treatment compared with control group,Y-27632 pretreatmen could prevent the permeability of BBB induced by TNF-?(P
