Regulatory Effect of Nitric Oxide on Hydrogen Sulfide/ Cystathionine-?-Lyase of Vascular Smooth Muscle Cells in Spontaneously Hypertensive Rats
- VernacularTitle:一氧化氮对自发性高血压大鼠血管平滑肌细胞硫化氢/胱硫醚-?-裂解酶的调节作用
- Author:
hong-fang, JIN
;
jun-bao, DU
;
chao-shu, TANG
;
ding-fang, BU
- Publication Type:Journal Article
- Keywords:
nitric oxide;
hydrogen sulfide;
cystathionine-?-lyase;
hypertension;
smooth muscle cells
- From:Journal of Applied Clinical Pediatrics
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nitric oxide on hydrogen sulfide/ cystathionine-?-lyase(CSE) system in the vascular smooth muscle cells of spontaneously hypertensive rats(SHR).Methods The SHR aortic smooth muscle cells(SMCs) were divided into 2 groups: sodium nitroprusside(SNP) group and control group.The content of hydrogen sulfide in the culture media was measured by sulfide electrode method,and the CSE mRNA level was assayed by competitive reversed transcription-polymerase chain reaction((RT-PCR)).Results Compared with control group,the content of hydrogen sulfide in the culture media of SNP group was significantly higher [(16.16?3.71) ?mol/L vs(22.71?4.84) ?mol/L],and the CSE mRNA level in SMCs in SNP group was lower than that of control group.Conclusion Nitric oxide exerted complicated effect on the hydrogen sulfide/CSE system in the SHR smooth muscle cells.J Appl Clin Pediatr,2006,21(3):140-141
