Expression Levels of Interleukin-12 and Interleukin-12 mRNA in Gastritis Related Infection with Helicobacter Pylori in Children
- VernacularTitle:幽门螺杆菌相关性胃炎患儿胃黏膜白细胞介素-12、白细胞介素-12信使核糖核酸的变化
- Author:
qian, LIN
;
da-wei, WANG
;
ying-zi, TANG
;
li-hua, HAO
;
mei, LI
;
zu-hui, HE
- Publication Type:Journal Article
- Keywords:
helicobacter pylori;
interleukin-12;
gastritis;
children
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the changes of interleukin-12(IL-12) and IL-12 mRNA in gastric mucosa of children with helicobacer pylori (Hp) infection,and to study the effects of Hp infection on the expression levels of IL-12 and IL-12 mRNA,and to evaluate its possible roles in the pathogenesis of gastric mucosal inflammation in Hp related gastroduodenal diseases.Methods Biopsy specimens were taken from the antral mucosa on endoscopy in patients with or without Hp infection, which were diagnosed by urease test and Giemsa staining. The expression levels of IL-12 and IL-12 mRNA in gastric mucosa were determined by ELISA and RT-PCR.Results Inflammation of gastric antral mucosa was more severe in Hp-positive mucosa .The expression levels of IL-12 and IL-12 mRNA in Hp-positive mucosa were (66.42?15.15) ng/g and (59.21?15.03)%,which were more than those in (Hp-negative )(22.22?8.79) ng/g and (17.94?7.39)%(P
