Detecting Hepatitis B Virus Deoxyribonucleic Acid Gene in Breast Milk by Nested Polymerase Chain Reaction
- VernacularTitle:套式聚合酶链反应技术检测母乳中乙肝病毒
- Author:
xiao-mei, YU
;
wei-hong, HU
;
jian-ming, DU
;
yin, ZHANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
deoxyribonucleic acid;
breast milk;
polymerase chain reaction
- From:Journal of Applied Clinical Pediatrics
2004;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective With the method of detecting hepatitis B virus deoxyribonucleic acid(HBV-DNA) gene in breast milk,in ordered to diagnose whether there is HBV in breast milk in those mothers whose HBsAg are positive.Methods Collected breast milk from 187 mothers whose HBsAg were positive,and complified HBV-N gene in breast milk by Nested-Polymerase chain reaction (Nested-PCR).Results Nested-PCR could detect HBV-DNA in breast milk,and the positive rate was 3.2%.Conclusion The method of detecting HBV-N gene in breast milk by Nested-PCR can detect HBV-DNA in breast milk,and it can be an laboratory evidence for whether breast feeding or not.
